CDK7 (Phospho-T170) polyclonal antibody

Product Specifications

Background

Cdk7 is a member of the cyclin dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of Saccharomyces cerevisiae cdc28, and Schizosaccharomyces pombe cdc2, and are known to be important regulators of cell cycle progression. Cdk7 forms a trimeric complex with cyclin H and MAT1, which functions as a Cdk activating kinase (CAK) . It is an essential component of the transcription factor TFIIH, that is involved in transcription initiation and DNA repair. This protein is thought to serve as a direct link between the regulation of transcription and the cell cycle.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Cyclin-dependent kinase 7; 39 kDa protein kinase; p39 Mo15; CDK-activating kinase 1; Cell division protein kinase 7; Serine/threonine-protein kinase 1; TFIIH basal transcription factor complex kinase subunit; CDK7; CAK; CAK1; CDKN7; MO15; STK1

Swiss Prot

P50613

Reactivity

Human, Mouse

Immunogen

Synthetic phosphopeptide derived from human CDK7 around the phosphorylation site of Threonine 170.

Conjugation

Unconjugated

Applications

WB IHC

Dilution

WB:1:500~1:1000 IHC:1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Phosphorylation

Molecular Weight

~ 40 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

CDK7 (Phospho-T170) polyclonal antibody detects endogenous levels of CDK7 protein only when phosphorylated at Thr170.

Applications Notes

Western blot (WB) analysis of CDK7 (Phospho-T170) polyclonal antibody at 1:500 dilution LaneA:Hela whole cell lysate LaneB:HEK293T whole cell lysate LaneC:A549 whole cell lysate LaneD:MCF-7 whole cell lysate LaneE:Raw264.7 whole cell lysate LaneF:The Spleen tissue lysate of Mouse