C/EBP epsilon (Phospho-T74) polyclonal antibody

Product Specifications

Background

C/EBP α regulates gene expression in a variety of tissues including liver, adipose, lung and intestine. C/EBP α uses a bipartite structural motif to bind DNA. Two protein chains dimerize through a set of amphipathic α helices termed the leucine zipper. Highly basic polypeptide regions emerge from the zipper to form a linked set of DNA contact surfaces. C/EBP α appears to function exclusively in terminally-differentiated, growth-arrested cells. Additional family members include C/EBP β, C/EBP γ, C/EBP δ and C/EBP ε, all of which exhibit similar DNA-binding specificities and affinities to C/EBP α. Furthermore, C/EBP β and C/EBP δ readily form heterodimers both with each other as well as with C/EBP α.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

CCAAT/enhancer-binding protein epsilon; C/EBP epsilon; CEBPE; C/EBPε

Swiss Prot

Q15744

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic phosphopeptide derived from human C/EBP epsilon around the phosphorylation site of Threonine 74.

Conjugation

Unconjugated

Applications

WB IHC IF

Dilution

WB:1:500~1:1000 IHC:1:50~1:200 IF:1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Phosphorylation

Molecular Weight

~ 34 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

C/EBP epsilon (Phospho-T74) polyclonal antibody detects endogenous levels of C/EBP epsilon protein only when phosphorylated at Thr74.

Applications Notes

Western blot (WB) analysis of C/EBP epsilon (Phospho-T74) polyclonal antibody at 1:500 dilution LaneA:Hela whole cell lysate LaneB:The Liver tissue lysate of Rat LaneC:Raw264.7 whole cell lysate LaneD:The Liver tissue lysate of Rat