MK2 (Phospho-T222) polyclonal antibody

Product Specifications

Background

In response to cytokines, stress, and chemotactic factors, MAP kinase-activated protein kinase 2 (MAPKAPK-2) is rapidly phosphorylated and activated. It has been shown that MAPKAPK-2 is a direct target of p38 MAPK. Multiple residues of MAPKAPK-2 are phosphorylated in vivo in response to stress. However, only four residues (Thr25, Thr222, Ser272, and Thr334) are phosphorylated by p38 MAPK in an in vitro kinase assay. Phosphorylation at Thr222, Ser272, and Thr334 appears to be essential for the activity of MAPKAPK-2. Thr25 is phosphorylated by p42 MAPK in vitro, but is not required for the activation of MAPKAPK-2.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

MAP kinase-activated protein kinase 2; MAPK-activated protein kinase 2; MAPKAP kinase 2; MAPKAP-K2; MAPKAPK-2; MK-2; MK2; MAPKAPK2

Swiss Prot

P49137

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic phosphopeptide derived from human MK2 around the phosphorylation site of Threonine 222.

Conjugation

Unconjugated

Related Pathways

Regulation of Microtubule Dynamics, Signaling Pathways Activating p38 MAP Kinase

Applications

WB IHC

Dilution

WB:1:500~1:1000 IHC:1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Phosphorylation

Molecular Weight

~ 49 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

MK2 (Phospho-T222) polyclonal antibody detects endogenous levels of MK2 protein only when phosphorylated at Thr222.

Applications Notes

Western blot (WB) analysis of MK2 (Phospho-T222) polyclonal antibody at 1:500 dilution LaneA:U-87MG whole cell lysate LaneB:K562 whole cell lysate LaneC:The Liver tissue lysate of Mouse LaneD:The Liver tissue lysate of Rat