AKT (Phospho-Y474) polyclonal antibody
Product Specifications
Background
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Swiss Prot
P31749
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic phosphopeptide derived from human AKT around the phosphorylation site of Tyrosine 474.
Conjugation
Unconjugated
Related Pathways
Phosphoinosotode Signaling, Protein Kinase C Signaling, Immune Cell Signaling, Warburg Effect, Insulin &Glucose Signaling, ErbB HER Signaling
Applications
WB IHC
Dilution
WB:1:500~1:1000 IHC:1:50~1:200
Purity
Modification
Phosphorylation
Molecular Weight
~ 60 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
AKT (Phospho-Y474) polyclonal antibody detects endogenous levels of AKT protein only when phosphorylated at Tyr474.
Applications Notes
Western blot (WB) analysis of AKT (Phospho-Y474) polyclonal antibody at 1:500 dilution LaneA:Zebrafish
Host or Source
Rabbit
Available Sizes
Frequently Asked Questions
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