CTnI (Phospho-S22/S23) polyclonal antibody

Product Specifications

Background

Troponin I is part of a heteromeric complex playing an important role in the regulation of skeletal and cardiac muscle contraction. It consists of three subunits, troponin I (TnI), troponin T (TnT) and troponin C (TnC) . Each subunit is responsible for part of troponin complex function. TnI inhibits ATPase activity of acto myosin and TnT and TnI are present in cardiac muscles in different forms than in skeletal muscles. Only one tissue specific isoform of TnI is described for cardiac muscle tissue (cTnI) and this is expressed only in myocardium.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Troponin I, cardiac muscle; Cardiac troponin I; TNNI3; TNNC1

Swiss Prot

P19429

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic phosphopeptide derived from human cTnI around the phosphorylation site of Serine 22/Serine 23.

Conjugation

Unconjugated

Applications

WB IHC

Dilution

WB:1:500~1:1000 IHC:1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Phosphorylation

Molecular Weight

~ 28 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

CTnI (Phospho-S22/S23) polyclonal antibody detects endogenous levels of cTnI protein only when phosphorylated at Ser22/Ser23.

Applications Notes

Western blot (WB) analysis of cTnI (Phospho-S22/S23) polyclonal antibody at 1:500 dilution LaneA:The Heart tissue lysate of Mouse LaneB:The Kidney tissue lysate of Mouse LaneC:The Heart tissue lysate of Rat LaneD:The Kidney tissue lysate of Rat