CaMK2 alpha/beta/delta (Phospho-T305) polyclonal antibody

Product Specifications

Background

The Ca2+/calmodulin-dependent protein kinases (CaM kinases) comprise a structurally related subfamily of serine/threonine kinases which include CaMKI, CaMKII and CaMKIV. CaMKII is an ubiquitously expressed serine/threonine protein kinase that is activated by Ca2+ and calmodulin (CaM) and has been implicated in regulation of the cell cycle and transcription. There are four CaMKII isozymes, designated α, β, γ and δ, which may or may not be co-expressed in the same tissue type. CaMKIIα is autophosphorylated on Thr 286 upon the binding of the Ca2+/CaM complex to the autoinhibitory domain of CaMKII. This process is called Ca2+/CaM trapping, which is thought to be involved in the synaptic encoding of information.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Calcium/calmodulin-dependent protein kinase type II subunit alpha; CaM kinase II subunit alpha; CaMK-II subunit alpha; CAMK2A; CAMKA; KIAA0968; CaMK2-a; CaMK2a; CaMK-2a; Calcium/calmodulin-dependent protein kinase type II subunit delta; CaM kinase II subunit delta; CaMK-II subunit delta; CAMK2D; CAMKD; CaMK2-δ; CaMK2δ; CaMK-2δ

Swiss Prot

Q9UQM7/Q13554/Q13557

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic phosphopeptide derived from human CaMK2 alpha/beta/delta around the phosphorylation site of Threonine 305.

Conjugation

Unconjugated

Related Pathways

Phospholipase Signaling, Phospholipase Signaling, Regulation of Microtubule Dynamics, Contribution of Soluble Factors to EMT, G Protein-coupled Receptors Signaling to MAPK Erk, G Protein-coupled Receptors Signaling to MAPK Erk

Applications

WB IHC

Dilution

WB:1:500~1:1000 IHC:1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Phosphorylation

Molecular Weight

~ 50 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

CaMK2 alpha/beta/delta (Phospho-T305) polyclonal antibody detects endogenous levels of CaMK2 alpha/beta/delta protein only when phosphorylated at Thr305.

Applications Notes

Western blot (WB) analysis of CaMK2 alpha/beta/delta (Phospho-T305) polyclonal antibody at 1:500 dilution LaneA:HEK293T whole cell lysate LaneB:U-87MG whole cell lysate LaneC:A549 whole cell lysate LaneD:The Brain tissue lysate of Rat