β-Actin (4D3) monoclonal antibody-HRP

Actin, a ubiquitous eukaryotic protein, is the major component of the cytoskeleton. At least six isoforms are known in mammals. Nonmuscle β- and γ-actin, also known as cytoplasmic actin, are predominantly expressed in nonmuscle cells, controlling cell structure and motility. α-cardiac and α-skeletal actin are expressed in striated cardiac and skeletal muscles, respectively; two smooth muscle actins, α- and γ-actin, are found primarily in vascular smooth muscle and enteric smooth muscle, respectively. These actin isoforms regulate the contractile potential of muscle cells.

The antibody was affinity-purified from mouse antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Western blot (WB) analysis of β-Actin (4D3) mAb-HRP at 1:2000/1:5000/1:10000 dilution Lane1:Hela whole cell lysate (40ug) Lane2:PC12 whole cell lysate (40ug) Lane3:NIH-3T3 whole cell lysate (40ug)