GAPDH (1A6) monoclonal antibody

Product Specifications

Background

Glyceraldehyde 3 phosphate dehydrogenase (GAPDH) is well known as one of the key enzymes involved in glycolysis. As well as functioning as a glycolytic enzyme in cytoplasm, recent evidence suggests that mammalian GAPDH is also involved in a great number of intracellular proceses such as membrane fusion, microtubule bundling, phosphotransferase activity, nuclear RNA export, DNA replication, and DNA repair. During the last decade a lot of data appeared concerning the role of GAPDH in different pathologies including prostate cancer progression, programmed neuronal cell death, age related neuronal diseases, such as Alzheimer's and Huntington's disease. GAPDH is expressed in all cells. It is constitutively expressed in almost all tissues at high levels.

CAS Number

9007-83-4

Structure Composition

1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Glyceraldehyde-3-phosphate dehydrogenase; GAPDH; GAPD

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant full length Human GAPDH.

Conjugation

Unconjugated

Applications

ELISA WB IHC IF

Dilution

ELISA: 1:50000~1:100000 WB: 1:10000 IHC:1:500~1:1000 IF:1:500~1:1000

Purity

The antibody was affinity-purified from mouse antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 36 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

GAPDH (1A6) mAb detects endogenous levels of GAPDH protein.

Applications Notes

Western blot (WB) analysis of GAPDH (1A6) mAb at 1:20000/1:10000 dilution Lane1:L02 whole cell lysate (40ug) Lane2:A549 whole cell lysate (40ug) Lane3:MG63 whole cell lysate (40ug) Lane4:PC12 whole cell lysate (40ug) Lane5:BV2 whole cell lysate (40ug) Lane6:The Brain tissue lysate of Rat (40ug) Lane7:The Brain tissue lysate of Mouse (40ug)