P-MSN; RDX; EZR (T558) polyclonal antibody

Product Specifications

Background

The ezrin, radixin, and moesin (ERM) proteins function as linkers between the plasma membrane and the actin cytoskeleton and are involved in cell adhesion, membrane ruffling, and microvilli formation. ERM proteins undergo intra or intermolecular interaction between their amino- and carboxy-terminal domains, existing as inactive cytosolic monomers or dimers. Phosphorylation at a carboxy-terminal threonine residue (Thr567 of ezrin, Thr564 of radixin, Thr558 of moesin) disrupts the amino- and carboxy-terminal association and may play a key role in regulating ERM protein conformation and function. Phosphorylation at Thr567 of ezrin is required for cytoskeletal rearrangements and oncogene-induced transformation. Ezrin is also phosphorylated at tyrosine residues upon growth factor stimulation. Phosphorylation of Tyr353 of ezrin transmits a survival signal during epithelial differentiation.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.

Product Name Alternative

Moesin; Membrane-organizing extension spike protein; MSN; Radixin; RDX; Ezrin; Cytovillin; Villin-2; p81; EZR

Swiss Prot

P26038; P35241; P15311

Reactivity

Human, Rat, Mouse

Immunogen

Synthetic peptide, corresponding to Human MSN; RDX; EZR (phospho-T558) .

Conjugation

Unconjugated

Related Pathways

Contribution of ECM and Cytoskeletal Factors to EMT, Contribution of ECM and Cytoskeletal Factors to EMT

Applications

Dilution

WB: 1:500~1:1000

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 75,80 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

MSN; RDX; EZR (phospho-T558) polyclonal antibody detects endogenous levels of MSN; RDX; EZR (phospho-T558) protein.

Applications Notes

Western blot (WB) analysis of MSN; RDX; EZR (phospho-T558) polyclonal antibody at 1:500 dilution Lane1:THP-1whole cell lysate (40ug) Lane2:H9C2 whole cell lysate (40ug) Lane3:The heart tissue lysate of Rat (40ug) Lane4:MCF-7 whole cell lysate (40ug) Lane5:A549 whole cell lysate (40ug) Lane6:SP2/0 whole cell lysate (40ug) Lane7:The lung tissue lysate of Rat (40ug)