MRPL16 polyclonal antibody

Product Specifications

Background

Mitochondrial ribosomal protein L16 (MRPL16) Homo sapiens Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. [provided by RefSeq, Jul 2008]

CAS Number

9007-83-4

Structure Composition

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Product Name Alternative

MRPL16; PNAS-111; 39S ribosomal protein L16; mitochondrial; L16mt; MRP-L16

Swiss Prot

Q9NX20

Reactivity

Human

Immunogen

The antiserum was produced against synthesized peptide derived from human MRPL16. AA range:171-220

Conjugation

Unconjugated

Applications

WB, IHC-p, IF (paraffin section), ELISA

Dilution

Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Modification

Unmodification

Molecular Weight

~ 28 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

MRP-L16 Polyclonal Antibody detects endogenous levels of MRP-L16 protein.

Applications Notes

Western Blot analysis of various cells using MRP-L16 Polyclonal Antibody diluted at 1: 2000

Host or Source

Rabbit

Available Sizes

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