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Epsin 3 Polyclonal Antibody

Product Specifications

Background

Induction:In keratinocytes, by wounding or contact with collagen., similarity:Belongs to the epsin family., similarity:Contains 1 ENTH (epsin N-terminal homology) domain., similarity:Contains 2 UIM (ubiquitin-interacting motif) repeats., subcellular location:Concentrated in the perinuclear region and associated with clathrin-coated vesicles close to the cell periphery. May shuttle to the nucleus., tissue specificity:Detected in migrating keratinocytes from wounded skin, but not in differentiating keratinocytes or in normal skin. Detected in chronic wounds, basal cell carcinoma and ulcerative colitis.

CAS Number

9007-83-4

Structure Composition

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Product Name Alternative

EPN3; Epsin-3; EPS-15-interacting protein 3

Swiss Prot

Q9H201

Reactivity

Human

Immunogen

The antiserum was produced against synthesized peptide derived from human EPN3. AA range:401-450

Conjugation

Unconjugated

Applications

WB, IHC-p, IF (paraffin section), ELISA

Dilution

Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Modification

Unmodification

Molecular Weight

~68 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

Epsin 3 Polyclonal Antibody detects endogenous levels of Epsin 3 protein.

Applications Notes

Western blot analysis of lysates from K562 cells, using EPN3 Antibody. The lane on the right is blocked with the synthesized peptide.

Host or Source

Rabbit

Available Sizes

Frequently Asked Questions

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