Claudin-7 (phospho Tyr210) Polyclonal Antibody

Product Specifications

Background

Claudin 7 (CLDN7) Homo sapiens This gene encodes a member of the claudin family. Claudins are integral membrane proteins and components of tight junction strands. Tight junction strands serve as a physical barrier to prevent solutes and water from passing freely through the paracellular space between epithelial or endothelial cell sheets, and also play critical roles in maintaining cell polarity and signal transductions. Differential expression of this gene has been observed in different types of malignancies, including breast cancer, ovarian cancer, hepatocellular carcinomas, urinary tumors, prostate cancer, lung cancer, head and neck cancers, thyroid carcinomas, etc.. Alternatively spliced transcript variants encoding different isoforms have been found.[provided by RefSeq, May 2010]

CAS Number

9007-83-4

Structure Composition

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Product Name Alternative

CLDN7; CEPTRL2; CPETRL2; Claudin-7; CLDN-7

Swiss Prot

O95471

Reactivity

Human, Mouse, Rat

Immunogen

The antiserum was produced against synthesized peptide derived from human Claudin 7 around the phosphorylation site of Tyr210. AA range:162-211

Conjugation

Unconjugated

Applications

WB, ELISA

Dilution

Western Blot: 1/500 - 1/2000. ELISA: 1/5000. Not yet tested in other applications.

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Modification

Phosphorylation

Molecular Weight

~32 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

Phospho-Claudin-7 (Y210) Polyclonal Antibody detects endogenous levels of Claudin-7 protein only when phosphorylated at Y210.

Applications Notes

Western blot analysis of lysates from rat liver, using Claudin 7 (Phospho-Tyr210) Antibody. The lane on the right is blocked with the phospho peptide.