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MRP-L47 Polyclonal Antibody

Product Specifications

Background

Mitochondrial ribosomal protein L47 (MRPL47) Homo sapiens Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. This gene is immediately adjacent to the gene for BAF complex 53 kDa subunit protein a (BAF53a), in a tail-to-tail orientation. Two transcript variants encoding different protein isoforms have been identifie

CAS Number

9007-83-4

Structure Composition

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Product Name Alternative

MRPL47; NCM1; CGI-204; 39S ribosomal protein L47; mitochondrial; L47mt; MRP-L47; Nasopharyngeal carcinoma metastasis-related protein 1

Swiss Prot

Q9HD33

Reactivity

Human

Immunogen

The antiserum was produced against synthesized peptide derived from human MRPL47. AA range:191-240

Conjugation

Unconjugated

Applications

WB, ELISA

Dilution

Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Modification

Unmodification

Molecular Weight

~30 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

MRP-L47 Polyclonal Antibody detects endogenous levels of MRP-L47 protein.

Applications Notes

Western blot analysis of lysates from RAW264.7 and K562 cells, using MRPL47 Antibody. The lane on the right is blocked with the synthesized peptide.

Host or Source

Rabbit

Available Sizes

Frequently Asked Questions

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