LYAR polyclonal antibody

Product Specifications

Background

LYAR, also named as ZLYAR, is 379 amino acid protein, which contains 2 C2H2-type zinc fingers. LYAR is expressed at at high levels in immature spermatocytes, early embryos and in fetal liver and thymus , with low expression in kidney and spleen. It is suggested that LYAR participates in regulating the stability of C23, a protein that is critical for maintaining the self-renewal and differentiation of embryonic stem cells.

CAS Number

9007-83-4

Structure Composition

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Product Name Alternative

LYAR; PNAS-5; Cell growth-regulating nucleolar protein

Swiss Prot

Q9NX58

Reactivity

Human

Immunogen

The antiserum was produced against synthesized peptide derived from human LYAR. AA range:241-290

Conjugation

Unconjugated

Applications

WB, IHC-p, IF (paraffin section), ELISA

Dilution

Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Modification

Unmodification

Molecular Weight

~ 44 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

LYAR Polyclonal Antibody detects endogenous levels of LYAR protein.

Applications Notes

Western Blot analysis of various cells using LYAR Polyclonal Antibody diluted at 1: 2000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA) .