MRPL10 polyclonal antibody

Product Specifications

Background

Mitochondrial ribosomal protein L10 (MRPL10) Homo sapiens Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. Sequence analysis identified three transcript variants that encode two different isoforms. A pseudogene corresponding to this gene is found on chromosome 5q. [provided by RefSeq, Nov 2010]

CAS Number

9007-83-4

Structure Composition

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Product Name Alternative

MRPL10; MRPL8; RPML8; 39S ribosomal protein L10; mitochondrial; L10mt; MRP-L10; 39S ribosomal protein L8, mitochondrial; L8mt; MRP-L8

Swiss Prot

Q7Z7H8

Reactivity

Human

Immunogen

The antiserum was produced against synthesized peptide derived from human MRPL10. AA range:211-260

Conjugation

Unconjugated

Applications

WB, IHC-p, IF (paraffin section), ELISA

Dilution

Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Modification

Unmodification

Molecular Weight

~ 29 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

MRP-L10 Polyclonal Antibody detects endogenous levels of MRP-L10 protein.

Applications Notes

Western Blot analysis of various cells using MRP-L10 Polyclonal Antibody diluted at 1: 1000

Host or Source

Rabbit

Available Sizes

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