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TIF-IA (Ab-649) polyclonal antibody

Product Specifications

Background

Function:Required for efficient transcription initiation by RNA polymerase I., similarity:Belongs to the RRN3 family.

CAS Number

9007-83-4

Structure Composition

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Product Name Alternative

RRN3; TIFIA; RNA polymerase I-specific transcription initiation factor RRN3; Transcription initiation factor IA; TIF-IA

Swiss Prot

Q9NYV6

Reactivity

Human

Immunogen

The antiserum was produced against synthesized peptide derived from human TIF-IA around the phosphorylation site of Ser649. AA range:602-651

Conjugation

Unconjugated

Applications

WB, IHC-p, IF (paraffin section), ELISA

Dilution

Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Modification

Unmodification

Molecular Weight

~ 70 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For

Specificity

Phospho-Rrn3 (S649) Polyclonal Antibody detects endogenous levels of Rrn3 protein only when phosphorylated at S649.

Applications Notes

Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using TIF-IA (Phospho-Ser649) Antibody

Host or Source

Rabbit

Available Sizes

Frequently Asked Questions

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