CA12 polyclonal antibody

Product Specifications

Background

Carbonic anhydrase 12 (CA12) Homo sapiens Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. This gene product is a type I membrane protein that is highly expressed in normal tissues, such as kidney, colon and pancreas, and has been found to be overexpressed in 10% of clear cell renal carcinomas. Three transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Jun 2014]

CAS Number

9007-83-4

Structure Composition

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Product Name Alternative

CA12; Carbonic anhydrase 12; Carbonate dehydratase XII; Carbonic anhydrase XII; CA-XII; Tumor antigen HOM-RCC-3.1.3

Swiss Prot

O43570

Reactivity

Human

Immunogen

The antiserum was produced against synthesized peptide derived from human CA12. AA range:231-280

Conjugation

Unconjugated

Applications

WB, IHC-p, IF (paraffin section), ELISA

Dilution

IHC-p: 100-300.Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Modification

Unmodification

Molecular Weight

~ 39 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For

Specificity

CA XII Polyclonal Antibody detects endogenous levels of CA XII protein.

Applications Notes

Western Blot analysis of NIH-3T3 293T cells using CA XII Polyclonal Antibody diluted at 1: 2000

Host or Source

Rabbit

Available Sizes

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