Caldesmon (Ab-759) polyclonal antibody

Caldesmon 1 (CALD1) Homo sapiens This gene encodes a calmodulin- and actin-binding protein that plays an essential role in the regulation of smooth muscle and nonmuscle contraction. The conserved domain of this protein possesses the binding activities to Ca (2+) -calmodulin, actin, tropomyosin, myosin, and phospholipids. This protein is a potent inhibitor of the actin-tropomyosin activated myosin MgATPase, and serves as a mediating factor for Ca (2+) -dependent inhibition of smooth muscle contraction. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2008]

This antibody detects endogenous levels of human Caldesmon. Heat-induced epitope retrieval (HIER) TRIS-EDTA of pH8.0 was highly recommended as antigen repair method in paraffin section

Immunohistochemical analysis of paraffin-embedded Liomyoma. 1, Antibody was diluted at 1:200 (4° overnight) . 2, Tris-EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min) .