P70 S6 Kinase β (Ab-423) polyclonal antibody

Product Specifications

Background

Ribosomal protein S6 kinase B2 (RPS6KB2) Homo sapiens This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains a kinase catalytic domain and phosphorylates the S6 ribosomal protein and eukaryotic translation initiation factor 4B (eIF4B) . Phosphorylation of S6 leads to an increase in protein synthesis and cell proliferation. [provided by RefSeq, Jan 2015]

CAS Number

9007-83-4

Structure Composition

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Product Name Alternative

RPS6KB2; STK14B; Ribosomal protein S6 kinase beta-2; S6K-beta-2; S6K2; 70 kDa ribosomal protein S6 kinase 2; P70S6K2; p70-S6K 2; S6 kinase-related kinase; SRK; Serine/threonine-protein kinase 14B; p70 ribosomal S6 kinase beta; S6K-beta; p70

Swiss Prot

Q9UBS0

Reactivity

Human

Immunogen

The antiserum was produced against synthesized peptide derived from human p70 S6 Kinase beta around the phosphorylation site of Ser423. AA range:389-438

Conjugation

Unconjugated

Applications

WB, ELISA

Dilution

Western Blot: 1/500 - 1/2000. ELISA: 1/5000. Not yet tested in other applications.

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Modification

Unmodification

Molecular Weight

~ 53 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For

Specificity

Phospho-p70 S6 kinase β (S423) Polyclonal Antibody detects endogenous levels of p70 S6 kinase β protein only when phosphorylated at S423.

Applications Notes

Western blot analysis of lysates from K562 cells treated with EGF 200ng/ml 5', using p70 S6 Kinase beta (Phospho-Ser423) Antibody. The lane on the right is blocked with the phospho peptide.