NMDAR2B (Ab-1336) polyclonal antibody

Product Specifications

Background

Glutamate ionotropic receptor NMDA type subunit 2A (GRIN2A) Homo sapiens This gene encodes a member of the glutamate-gated ion channel protein family. The encoded protein is an N-methyl-D-aspartate (NMDA) receptor subunit. NMDA receptors are both ligand-gated and voltage-dependent, and are involved in long-term potentiation, an activity-dependent increase in the efficiency of synaptic transmission thought to underlie certain kinds of memory and learning. These receptors are permeable to calcium ions, and activation results in a calcium influx into post-synaptic cells, which results in the activation of several signaling cascades. Disruption of this gene is associated with focal epilepsy and speech disorder with or without mental retardation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2014]

CAS Number

9007-83-4

Structure Composition

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Product Name Alternative

GRIN2A; NMDAR2A; Glutamate [NMDA] receptor subunit epsilon-1; N-methyl D-aspartate receptor subtype 2A; NMDAR2A; NR2A; hNR2A; GRIN2B; NMDAR2B; Glutamate [NMDA] receptor subunit epsilon-2; N-methyl D-aspartate receptor subtype 2B; NMDAR2B; N

Swiss Prot

Q13224

Reactivity

Human

Immunogen

The antiserum was produced against synthesized peptide derived from human NMDAR2A/B around the phosphorylation site of Tyr1246/1252. AA range:1216-1265

Conjugation

Unconjugated

Applications

WB, IHC-p, IF/ICC, ELISA

Dilution

Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Modification

Unmodification

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For

Specificity

Phospho-NMDAε1/2 (Y1246/1252) Polyclonal Antibody detects endogenous levels of NMDAε1/2 protein only when phosphorylated at Y1246/1252.

Applications Notes

Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100 (4° overnight) . High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.