HMG17 polyclonal antibody

Product Specifications

Background

High mobility group nucleosomal binding domain 2 (HMGN2) Homo sapiens The protein encoded by this gene binds nucleosomal DNA and is associated with transcriptionally active chromatin. Along with a similar protein, HMGN1, the encoded protein may help maintain an open chromatin configuration around transcribable genes. The protein has also been found to have antimicrobial activity against bacteria, viruses and fungi. [provided by RefSeq, Oct 2014]

CAS Number

9007-83-4

Structure Composition

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Product Name Alternative

HMGN2; HMG17; Non-histone chromosomal protein HMG-17; High mobility group nucleosome-binding domain-containing protein 2

Swiss Prot

P05204

Reactivity

Human

Immunogen

The antiserum was produced against synthesized peptide derived from human HMG17 around the phosphorylation site of Ser29. AA range:1-50

Conjugation

Unconjugated

Applications

IHC-p, IF (paraffin section), ELISA

Dilution

Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Modification

Unmodification

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For

Specificity

Phospho-HMG-17 (S29) Polyclonal Antibody detects endogenous levels of HMG-17 protein only when phosphorylated at S29.

Applications Notes

Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100 (4° overnight) . High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.