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GLI-3 polyclonal antibody

Product Specifications

Background

GLI family zinc finger 3 (GLI3) Homo sapiens This gene encodes a protein which belongs to the C2H2-type zinc finger proteins subclass of the Gli family. They are characterized as DNA-binding transcription factors and are mediators of Sonic hedgehog (Shh) signaling. The protein encoded by this gene localizes in the cytoplasm and activates patched Drosophila homolog (PTCH) gene expression. It is also thought to play a role during embryogenesis. Mutations in this gene have been associated with several diseases, including Greig cephalopolysyndactyly syndrome, Pallister-Hall syndrome, preaxial polydactyly type IV, and postaxial polydactyly types A1 and B. [provided by RefSeq, Jul 2008]

CAS Number

9007-83-4

Structure Composition

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Product Name Alternative

GLI3; Transcriptional activator GLI3; GLI3 form of 190 kDa; GLI3-190; GLI3 full length protein; GLI3FL

Swiss Prot

P10071

Reactivity

Human

Immunogen

The antiserum was produced against synthesized peptide derived from human GLI-3. AA range:11-60

Conjugation

Unconjugated

Related Pathways

Hedgehog Signaling

Applications

IHC-p, IF/ICC, ELISA

Dilution

Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Modification

Unmodification

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For

Specificity

GLI-3 Polyclonal Antibody detects endogenous levels of GLI-3 protein.

Applications Notes

Immunofluorescence analysis of HepG2 cells, using GLI-3 Antibody. The picture on the right is blocked with the synthesized peptide.

Host or Source

Rabbit

Available Sizes

Frequently Asked Questions

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