ACOT12 polyclonal antibody
Product Specifications
Background
Catalytic activity:Acetyl-CoA + H (2) O = CoA + acetate., function:Hydrolyzes acetyl-CoA to acetate and CoA., pathway:Carbohydrate metabolism; pyruvate metabolism., similarity:Contains 1 START domain., similarity:Contains 2 acyl coenzyme A hydrolase domains., subunit:Homodimer or homotetramer.
CAS Number
9007-83-4
Structure Composition
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Product Name Alternative
ACOT12; CACH; CACH1; STARD15; Acyl-coenzyme A thioesterase 12; Acyl-CoA thioesterase 12; Acyl-CoA thioester hydrolase 12; Cytoplasmic acetyl-CoA hydrolase 1; CACH-1; hCACH-1; START domain-containing protein 15; StARD15
Swiss Prot
Q8WYK0
Reactivity
Human
Immunogen
The antiserum was produced against synthesized peptide derived from human ACOT12. AA range:281-330
Conjugation
Unconjugated
Applications
IHC-p, IF/ICC, ELISA
Dilution
Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Modification
Unmodification
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For
Specificity
ACOT12 Polyclonal Antibody detects endogenous levels of ACOT12 protein.
Applications Notes
Immunohistochemical analysis of paraffin-embedded Human lung cancer. Antibody was diluted at 1:100 (4° overnight) . High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
Host or Source
Rabbit
Available Sizes
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