HMG14 (Phospho-Ser21) polyclonal antibody

Product Specifications

Background

High mobility group nucleosome binding domain 1 (HMGN1) Homo sapiens The protein encoded by this gene binds nucleosomal DNA and is associated with transcriptionally active chromatin. Along with a similar protein, HMG17, the encoded protein may help maintain an open chromatin configuration around transcribable genes. [provided by RefSeq, Aug 2011]

CAS Number

9007-83-4

Structure Composition

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Product Name Alternative

HMGN1; HMG14; Non-histone chromosomal protein HMG-14; High mobility group nucleosome-binding domain-containing protein 1

Swiss Prot

P05114

Reactivity

Human

Immunogen

The antiserum was produced against synthesized peptide derived from human HMG14 around the phosphorylation site of Ser21. AA range:10-59

Conjugation

Unconjugated

Applications

IHC-p, IF/ICC, ELISA

Dilution

Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Modification

Phosphorylation

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For

Specificity

Phospho-HMG-14 (S21) Polyclonal Antibody detects endogenous levels of HMG-14 protein only when phosphorylated at S21.

Applications Notes

Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100 (4° overnight) . High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.