CSGALNACT1 polyclonal antibody

Product Specifications

Background

Catalytic activity:UDP-N-acetyl-D-galactosamine + beta-D-glucuronyl- (1->3) -D-galactosyl-proteoglycan = UDP + N-acetyl-D-galactosaminyl- (1->4) -beta-D-glucuronyl- (1->3) -beta-D-galactosylproteoglycan., function:Transfers 1,4-N-acetylgalactosamine (GalNAc) from UDP-GalNAc to the non-reducing end of glucuronic acid (GlcUA) . Required for addition of the first GalNAc to the core tetrasaccharide linker and for elongation of chondroitin chains. Important role in chondroitin chain biosynthesis in cartilage., online information:Chondroitin beta-1,4-N-acetylgalactosaminyltransferase 1, online information:GlycoGene database, PTM:N-glycosylated., similarity:Belongs to the chondroitin N-acetylgalactosaminyltransferase family., tissue specificity:Ubiquitous, with the highest levels in placenta, thyroid, bladder, prostate and adrenal gland. Detected at low levels in the other tissues examined.

CAS Number

9007-83-4

Structure Composition

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Product Name Alternative

CSGALNACT1; CHGN; GALNACT1; Chondroitin sulfate N-acetylgalactosaminyltransferase 1; CsGalNAcT-1; Chondroitin beta-1; 4-N-acetylgalactosaminyltransferase 1; Beta4GalNAcT-1

Swiss Prot

Q8TDX6

Reactivity

Human, Mouse, Rat

Immunogen

The antiserum was produced against synthesized peptide derived from human CSGALNACT1. AA range:201-250

Conjugation

Unconjugated

Applications

WB, IHC-p, IF (paraffin section), ELISA

Dilution

Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Modification

Unmodification

Molecular Weight

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

β-1,4-GalNAc-T Polyclonal Antibody detects endogenous levels of β-1,4-GalNAc-T protein.

Applications Notes

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using CSGALNACT1 Antibody. The picture on the right is blocked with the synthesized peptide.