BUB1 polyclonal antibody

Product Specifications

Background

BUB1 mitotic checkpoint serine/threonine kinase (BUB1) Homo sapiens This gene encodes a serine/threonine-protein kinase that play a central role in mitosis. The encoded protein functions in part by phosphorylating members of the mitotic checkpoint complex and activating the spindle checkpoint. This protein also plays a role in inhibiting the activation of the anaphase promoting complex/cyclosome. This protein may also function in the DNA damage response. Mutations in this gene have been associated with aneuploidy and several forms of cancer. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]

CAS Number

9007-83-4

Structure Composition

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Product Name Alternative

BUB1; BUB1L; Mitotic checkpoint serine/threonine-protein kinase BUB1; hBUB1; BUB1A

Swiss Prot

O43683

Reactivity

Human

Immunogen

The antiserum was produced against synthesized peptide derived from human BUB1. AA range:781-830

Conjugation

Unconjugated

Applications

WB, ELISA

Dilution

Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Modification

Unmodification

Molecular Weight

~ 120 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

BUB1 Polyclonal Antibody detects endogenous levels of BUB1 protein.

Applications Notes

Western blot analysis of the lysates from HUVECcells using BUB1 antibody.

Host or Source

Rabbit

Available Sizes

Curated Selection

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