POU2F1/OCT1 (Phospho-Ser385) polyclonal antibody

Product Specifications

Background

POU class 2 homeobox 1 (POU2F1) Homo sapiens The OCT1 transcription factor was among the first identified members of the POU transcription factor family (summarized by Sturm et al., 1993 [PubMed 8314572]) . Members of this family contain the POU domain, a 160-amino acid region necessary for DNA binding to the octameric sequence ATGCAAAT.[supplied by OMIM, Jul 2010]

CAS Number

9007-83-4

Structure Composition

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Product Name Alternative

POU2F1; OCT1; OTF1; POU domain; class 2, transcription factor 1; NF-A1; Octamer-binding protein 1; Oct-1; Octamer-binding transcription factor 1; OTF-1; POU2F2; OCT2; OTF2; POU domain, class 2, transcription factor 2; Lymphoid-restricted im

Swiss Prot

P14859/P09086

Reactivity

Human, Mouse, Rat

Immunogen

The antiserum was produced against synthesized peptide derived from human POU2F1/OCT1 around the phosphorylation site of Ser385. AA range:361-410

Conjugation

Unconjugated

Applications

IHC-p, IF (paraffin section), ELISA

Dilution

Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Modification

Phosphorylation

Molecular Weight

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

Phospho-Oct-1 (S385) Polyclonal Antibody detects endogenous levels of 41183 protein only when phosphorylated at S385.

Applications Notes

Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using POU2F1/OCT1 (Phospho-Ser385) Antibody