SHC3 polyclonal antibody

Product Specifications

Background

Function:Signaling adapter that couples activated growth factor receptors to signaling pathway in neurons. Involved in the signal transduction pathways of neurotrophin-activated Trk receptors in cortical neurons., PTM:Tyrosine phosphorylated., similarity:Contains 1 PID domain., similarity:Contains 1 SH2 domain., subunit:Interacts with the Trk receptors in a phosphotyrosine-dependent manner. Once activated, binds to GRB2. Interacts with activated EGF receptors., tissue specificity:Mainly expressed in brain. Hardly detectable in other tissues, except in pancreas. Highly expressed in the cerebral cortex, frontal and temporal lobes, occipital pole, hippocampus, caudate nucleus and amygdala. Expressed at low level in the cerebellum, medulla and spinal cord.

CAS Number

9007-83-4

Structure Composition

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Product Name Alternative

SHC3; NSHC; SHCC; SHC-transforming protein 3; Neuronal Shc; N-Shc; Protein Rai; SHC-transforming protein C; Src homology 2 domain-containing-transforming protein C3; SH2 domain protein C3

Swiss Prot

Q92529

Reactivity

Human, Mouse, Rat

Immunogen

The antiserum was produced against synthesized peptide derived from human SHC3. AA range:291-340

Conjugation

Unconjugated

Applications

IHC-p, IF (paraffin section), ELISA

Dilution

Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Modification

Unmodification

Molecular Weight

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

N-Shc Polyclonal Antibody detects endogenous levels of N-Shc protein.

Applications Notes

Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using SHC3 Antibody. The picture on the right is blocked with the synthesized peptide.