MNK1 (Ab-250) polyclonal antibody

Product Specifications

Background

MAP kinase interacting serine/threonine kinase 1 (MKNK1) Homo sapiens This gene encodes a Ser/Thr protein kinase that interacts with, and is activated by ERK1 and p38 mitogen-activated protein kinases, and thus may play a role in the response to environmental stress and cytokines. This kinase may also regulate transcription by phosphorylating eIF4E via interaction with the C-terminal region of eIF4G. Alternatively spliced transcript variants have been noted for this gene. [provided by RefSeq, Jan 2012]

CAS Number

9007-83-4

Structure Composition

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Product Name Alternative

MKNK1; MNK1; MAP kinase-interacting serine/threonine-protein kinase 1; MAP kinase signal-integrating kinase 1; MAPK signal-integrating kinase 1; Mnk1

Swiss Prot

Q9BUB5

Reactivity

Human, Mouse, Rat

Immunogen

The antiserum was produced against synthesized peptide derived from human MNK1 around the phosphorylation site of Thr250. AA range:216-265

Conjugation

Unconjugated

Related Pathways

Phospholipase Signaling, Translational Contral elF4 and p70 S6 Kinase, Signaling Pathways Activating p38 MAP KinaseInsulin &Glucose Signaling

Applications

IHC-p, IF (paraffin section), ELISA

Dilution

Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Modification

Unmodification

Molecular Weight

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

Phospho-Mnk1 (T250) Polyclonal Antibody detects endogenous levels of Mnk1 protein only when phosphorylated at T250.

Applications Notes

Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using MNK1 (Phospho-Thr250) Antibody