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HIRA (Ab-555) polyclonal antibody

Product Specifications

Background

Histone cell cycle regulator (HIRA) Homo sapiens This gene encodes a histone chaperone that preferentially places the variant histone H3.3 in nucleosomes. Orthologs of this gene in yeast, flies, and plants are necessary for the formation of transcriptionally silent heterochomatin. This gene plays an important role in the formation of the senescence-associated heterochromatin foci. These foci likely mediate the irreversible cell cycle changes that occur in senescent cells. It is considered the primary candidate gene in some haploinsufficiency syndromes such as DiGeorge syndrome, and insufficient production of the gene may disrupt normal embryonic development. [provided by RefSeq, Jul 2008]

CAS Number

9007-83-4

Structure Composition

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Product Name Alternative

HIRA; DGCR1; HIR; TUPLE1; Protein HIRA; TUP1-like enhancer of split protein 1

Swiss Prot

P54198

Reactivity

Human, Mouse

Immunogen

The antiserum was produced against synthesized peptide derived from human HIRA around the phosphorylation site of Thr555. AA range:521-570

Conjugation

Unconjugated

Related Pathways

ATP-Dependent Chromatin Remodeling Complexes

Applications

WB, IHC-p, IF/ICC, ELISA

Dilution

Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Modification

Unmodification

Molecular Weight

/

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

Phospho-HIRA (T555) Polyclonal Antibody detects endogenous levels of HIRA protein only when phosphorylated at T555.

Applications Notes

Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100 (4° overnight) . High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Host or Source

Rabbit

Available Sizes

Frequently Asked Questions

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