IGFBP-3 (Phospho-Ser183) polyclonal antibody
Product Specifications
Background
CAS Number
9007-83-4
Structure Composition
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Product Name Alternative
IGFBP3; IBP3; Insulin-like growth factor-binding protein 3; IBP-3; IGF-binding protein 3; IGFBP-3
Swiss Prot
P17936
Reactivity
Human, Mouse
Immunogen
The antiserum was produced against synthesized peptide derived from human IGFBP-3 around the phosphorylation site of Ser183. AA range:151-200
Conjugation
Unconjugated
Applications
WB, IHC-p, IF (paraffin section), ELISA
Dilution
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Modification
Phosphorylation
Molecular Weight
~ 31 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
Phospho-IGFBP3 (S183) Polyclonal Antibody detects endogenous levels of IGFBP3 protein only when phosphorylated at S183.
Applications Notes
Western blot analysis of lysates from Jarkat cells, primary antibody was diluted at 1:1000, 4°over night
Host or Source
Rabbit
Available Sizes
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