ARHGDIA (Ab-174) polyclonal antibody

Product Specifications

Background

Rho GDP dissociation inhibitor alpha (ARHGDIA) Homo sapiens This gene encodes a protein that plays a key role in the regulation of signaling through Rho GTPases. The encoded protein inhibits the disassociation of Rho family members from GDP (guanine diphosphate), thereby maintaining these factors in an inactive state. Activity of this protein is important in a variety of cellular processes, and expression of this gene may be altered in tumors. Mutations in this gene have been found in individuals with nephrotic syndrome, type 8. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014]

CAS Number

9007-83-4

Structure Composition

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Product Name Alternative

ARHGDIA; GDIA1; Rho GDP-dissociation inhibitor 1; Rho GDI 1; Rho-GDI alpha

Swiss Prot

P52565

Reactivity

Human, Mouse, Rat

Immunogen

The antiserum was produced against synthesized peptide derived from human ARHGDIA around the phosphorylation site of Ser174. AA range:140-189

Conjugation

Unconjugated

Applications

WB, IHC-p, IF (paraffin section), ELISA

Dilution

Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Modification

Unmodification

Molecular Weight

~ 23 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

Phospho-Rho GDIα (S174) Polyclonal Antibody detects endogenous levels of Rho GDIα protein only when phosphorylated at S174.

Applications Notes

Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using ARHGDIA (Phospho-Ser174) Antibody