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PLK1 (Phospho-Thr210) polyclonal antibody

Product Specifications

Background

Polo like kinase 1 (PLK1) Homo sapiens The Ser/Thr protein kinase encoded by this gene belongs to the CDC5/Polo subfamily. It is highly expressed during mitosis and elevated levels are found in many different types of cancer. Depletion of this protein in cancer cells dramatically inhibited cell proliferation and induced apoptosis; hence, it is a target for cancer therapy. [provided by RefSeq, Sep 2015]

CAS Number

9007-83-4

Structure Composition

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Product Name Alternative

PLK1; PLK; Serine/threonine-protein kinase PLK1; Polo-like kinase 1; PLK-1; Serine/threonine-protein kinase 13; STPK13

Swiss Prot

P53350

Reactivity

Human, Mouse, Rat

Immunogen

The antiserum was produced against synthesized peptide derived from human PLK1 around the phosphorylation site of Thr210. AA range:176-225

Conjugation

Unconjugated

Applications

WB, IHC-p, IF (paraffin section), ELISA

Dilution

WB 1:500-2000 , Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Modification

Phosphorylation

Molecular Weight

~ 70 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

Phospho-Plk (T210) Polyclonal Antibody detects endogenous levels of Plk protein only when phosphorylated at T210.

Applications Notes

Western blot analysis of 293 using p-Plk (T210) antibody.

Host or Source

Rabbit

Available Sizes

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