TGF β Receptor II (Phospho-Ser225/250) polyclonal antibody
Product Specifications
Background
Transforming growth factor beta receptor 2 (TGFBR2) Homo sapiens This gene encodes a member of the Ser/Thr protein kinase family and the TGFB receptor subfamily. The encoded protein is a transmembrane protein that has a protein kinase domain, forms a heterodimeric complex with another receptor protein, and binds TGF-beta. This receptor/ligand complex phosphorylates proteins, which then enter the nucleus and regulate the transcription of a subset of genes related to cell proliferation. Mutations in this gene have been associated with Marfan Syndrome, Loeys-Deitz Aortic Aneurysm Syndrome, and the development of various types of tumors. Alternatively spliced transcript variants encoding different isoforms have been characterized. [provided by RefSeq, Jul 2008]
CAS Number
9007-83-4
Structure Composition
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Product Name Alternative
TGFBR2; TGF-beta receptor type-2; TGFR-2; TGF-beta type II receptor; Transforming growth factor-beta receptor type II; TGF-beta receptor type II; TbetaR-II
Swiss Prot
P37173
Reactivity
Human, Mouse
Immunogen
The antiserum was produced against synthesized peptide derived from human TGF beta Receptor II around the phosphorylation site of Ser225/250. AA range:191-240
Conjugation
Unconjugated
Related Pathways
Contribution of Soluble Factors to EMT
Applications
IHC-p, IF (paraffin section), ELISA
Dilution
WB 1:500-2000 , Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Modification
Phosphorylation
Molecular Weight
~ 65 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
Phospho-TGFβ RII (S225) Polyclonal Antibody detects endogenous levels of TGFβ RII protein only when phosphorylated at S225.
Applications Notes
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using TGF beta Receptor II (Phospho-Ser225/250) Antibody
Host or Source
Rabbit
Available Sizes
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