Bax (Phospho-Thr167) polyclonal antibody

Product Specifications

Background

The protein encoded by BAX (BCL2 associated X, apoptosis regulator) belongs to the BCL2 protein family. BCL2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein forms a heterodimer with BCL2, and functions as an apoptotic activator. This protein is reported to interact with, and increase the opening of, the mitochondrial voltage-dependent anion channel (VDAC), which leads to the loss in membrane potential and the release of cytochrome c. The expression of this gene is regulated by the tumor suppressor P53 and has been shown to be involved in P53-mediated apoptosis. Multiple alternatively spliced transcript variants, which encode different isoforms, have been reported for BAX

CAS Number

9007-83-4

Structure Composition

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Product Name Alternative

BAX; BCL2L4; Apoptosis regulator BAX; Bcl-2-like protein 4; Bcl2-L-4

Swiss Prot

Q07812

Reactivity

Human, Mouse, Rat

Immunogen

The antiserum was produced against synthesized peptide derived from human Bax around the phosphorylation site of Thr167. AA range:133-182

Conjugation

Unconjugated

Related Pathways

Regulation of Apoptosis, SAPK JNK Signaling Cascades, Inhibition of Apoptosis, Mitochondrial Control of Apoptosis

Applications

IF/ICC, ELISA

Dilution

Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Modification

Phosphorylation

Molecular Weight

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

Phospho-Bax (T167) Polyclonal Antibody detects endogenous levels of Bax protein only when phosphorylated at T167.

Applications Notes

Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using Bax (Phospho-Thr167) Antibody