MDM2 (phospho Ser186/S188) polyclonal antibody
Product Specifications
Background
CAS Number
9007-83-4
Structure Composition
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Product Name Alternative
MDM2; E3 ubiquitin-protein ligase Mdm2; Double minute 2 protein; Hdm2; Oncoprotein Mdm2; p53-binding protein Mdm2
Swiss Prot
Q00987
Reactivity
Human
Immunogen
The antiserum was produced against synthesized peptide derived from human MDM2 around the phosphorylation site of Ser186 and Ser188. AA range:151-200
Conjugation
Unconjugated
Related Pathways
Examples of Crosstalk Between Post-translational Modifications
Applications
IF/ICC, WB, ELISA
Dilution
WB 1:500-2000 Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Modification
Phosphorylation
Molecular Weight
/
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
Phospho-MDM2 (S186/S188) Polyclonal Antibody detects endogenous levels of MDM2 protein only when phosphorylated at S186/S188.
Applications Notes
Host or Source
Rabbit
Available Sizes
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