PARP2 polyclonal antibody

Product Specifications

Background

Involved in the base excision repair (BER) pathway, by catalyzing the poly (ADP-ribosyl) ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. Mediates serine ADP-ribosylation of target proteins following interaction with HPF1; HPF1 conferring serine specificity.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

PARP-2, hPARP-2, ARTD2, ADPRT-2, pADPRT-2, PARP2, ADPRT2, ADPRTL2, ADP ribosyltransferase like 2, ADP-ribosyltransferase diphtheria toxin-like 2, ADPRT 2, ADPRT-2, ADPRT2, ADPRTL 2, ADPRTL 3, ADPRTL2, ADPRTL3, ARTD2, hPARP 2, hPARP-2, hPARP2, NAD (+) ADP ribosyltransferase 2, NAD (+) ADP-ribosyltransferase 2, pADPRT 2, pADPRT-2, pADPRT2, PARP 2, PARP-2, PARP2, PARP2, Poly (ADP ribose) polymerase family member 2, Poly (ADP ribosyl) transferase like 2, poly (ADP-ribose) polymerase 2, Poly [ADP ribose] synthetase 2, Poly [ADP-ribose] polymerase 2, Poly (ADP ribose) synthetase, Poly[ADP-ribose] synthase 2

Swiss Prot

Q9UGN5

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide, corresponding to Human PARP2.

Conjugation

Unconjugated

Applications

Dilution

WB:1:1000~1:2000

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 66 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

PARP2 polyclonal antibody detects endogenous levels of PARP2 protein.

Applications Notes

Western blot (WB) analysis of PARP2 pAb at 1:1000 dilution Lane1:SHSY5Y whole cell lysate (40ug) Lane2:U-87MG whole cell lysate (40ug) Lane3:The heart tissue lysate of Rat (40ug) Lane4:H9C2 whole cell lysate (40ug)