Wilms Tumor Protein Polyclonal Antibody

Product Specifications

Background

Transcription factor that plays an important role in cellular development and cell survival. Regulates the expression of numerous target genes, including EPO. Plays an essential role for development of the urogenital system. Recognizes and binds to the DNA sequence 5'-CGCCCCCGC-3'. It has a tumor suppressor as well as an oncogenic role in tumor formation. Function may be isoform-specific: isoforms lacking the KTS motif may act as transcription factors. Isoforms containing the KTS motif may bind mRNA and play a role in mRNA metabolism or splicing. Isoform 1 has lower affinity for DNA, and can bind RNA.

CAS Number

9007-83-4

Structure Composition

0.01M TBS (pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Product Name Alternative

WIT 2; WT 1; AWT1; FWT1; GUD; NPHS4; WAGR; Wilms tumor 1; Wilms Tumor; Wilms tumor protein; Wilms' tumor gene; Wilms' tumor protein; WIT2; WT; WT1; WT-1; WT1_HUMAN; WT33.

Swiss Prot

P19544

Reactivity

Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Sheep

Immunogen

KLH conjugated synthetic peptide derived from human Wilms Tumor Protein:301-400/449

Conjugation

Unconjugated

Applications

IHC-P, FCM

Dilution

IHC-P=1:100-500 Flow-Cyt=1ug/test

Purity

Affinity purified by Protein A

Modification

Unmodification

Molecular Weight

55kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

Wilms Tumor Protein Polyclonal Antibody detects endogenous levels of Wilms Tumor Protein protein.

Applications Notes

Tissue/cell: human kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Incubation: Anti-WT-1/Wilms Tumor Protein Polyclonal Antibody, Unconjugated 1:200, overnight at 4°C