Thymidine phosphorylase Polyclonal Antibody

Product Specifications

Background

In the presence of inorganic orthophosphate, the platelet-derived endothelial growth factor (PD-ECGF) thymidine phosphorylase (TP) (gliostatin) catalyses the reversible phospholytic cleavage of thymidine and deoxyuridine to their corresponding bases and 2-deoxyribose-1-phosphate. It is both chemotactic and mitogenic for endothelial cells and a non-heparin binding angiogenic factor present in platelets. It is also involved in transformation of fluoropyrimidines, cytotoxic agents used in the treatment of a variety of malignancies, into active cytotoxic metabolites.

CAS Number

9007-83-4

Structure Composition

0.01M TBS (pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Product Name Alternative

ECGF 1; PDEGF; ECGF1; Endothelial cell growth factor 1; Endothelial cell growth factor 1 platelet derived; Gliostatin; hPD ECGF; MNGIE; PD ECGF; PDECGF; PDEGF; Platelet derived endothelial growth factor; TdRPase; TP; TYMP; Thymidine Phosphorylase; TYPH_HUMAN; TdRPase.

Swiss Prot

P19971

Reactivity

Human

Immunogen

KLH conjugated synthetic peptide derived from human Thymidine Phosphorylase:121-220/482

Conjugation

Unconjugated

Applications

IHC-P

Dilution

IHC-P=1:100-500

Purity

Affinity purified by Protein A

Modification

Phosphorylation

Molecular Weight

~50kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -42°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

Thymidine phosphorylase Polyclonal Antibody detects endogenous levels of thymidine phosphorylase protein.

Applications Notes

Paraformaldehyde-fixed, paraffin embedded (human gastric carcinoma) ; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TYPH) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C