Thymidine phosphorylase Polyclonal Antibody
Product Specifications
Background
In the presence of inorganic orthophosphate, the platelet-derived endothelial growth factor (PD-ECGF) thymidine phosphorylase (TP) (gliostatin) catalyses the reversible phospholytic cleavage of thymidine and deoxyuridine to their corresponding bases and 2-deoxyribose-1-phosphate. It is both chemotactic and mitogenic for endothelial cells and a non-heparin binding angiogenic factor present in platelets. It is also involved in transformation of fluoropyrimidines, cytotoxic agents used in the treatment of a variety of malignancies, into active cytotoxic metabolites.
CAS Number
9007-83-4
Structure Composition
0.01M TBS (pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Product Name Alternative
ECGF 1; PDEGF; ECGF1; Endothelial cell growth factor 1; Endothelial cell growth factor 1 platelet derived; Gliostatin; hPD ECGF; MNGIE; PD ECGF; PDECGF; PDEGF; Platelet derived endothelial growth factor; TdRPase; TP; TYMP; Thymidine Phosphorylase; TYPH_HUMAN; TdRPase.
Swiss Prot
P19971
Reactivity
Human
Immunogen
KLH conjugated synthetic peptide derived from human Thymidine Phosphorylase:121-220/482
Conjugation
Unconjugated
Applications
IHC-P
Dilution
IHC-P=1:100-500
Purity
Affinity purified by Protein A
Modification
Phosphorylation
Molecular Weight
~50kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -42°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
Thymidine phosphorylase Polyclonal Antibody detects endogenous levels of thymidine phosphorylase protein.
Applications Notes
Paraformaldehyde-fixed, paraffin embedded (human gastric carcinoma) ; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TYPH) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C
Host or Source
Rabbit
Available Sizes
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