Nestin Polyclonal Antibody
Product Specifications
Background
Nestin is a Class VI intermediate filament expressed in the developing central nervous system (CNS) in early embryonic neuroepithelial stem cells. This protein has been widely used as a predominant marker for stem / progenitor cells, glioma cells, and tumor endothelial cells in the mammalian CNS. Furthermore, it is a superior angiogenic marker to evaluate neovascularity of endothelial cells in tumor.
CAS Number
9007-83-4
Structure Composition
0.01M TBS (pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Product Name Alternative
ESTM 46; FLJ 21841; FLJ21841; Intermediate filament protein; Nbla00170; Nes; NEST_MOUSE; Nestin.
Swiss Prot
Q6P5H2
Reactivity
Mouse, Rat
Immunogen
KLH conjugated synthetic peptide derived from mouse Nestin:1231-1330/1864
Conjugation
Unconjugated
Applications
IHC-P
Dilution
IHC-P=1:100-500
Purity
Affinity purified by Protein A
Modification
Unmodification
Molecular Weight
~209kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -31°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
Nestin Polyclonal Antibody detects endogenous levels of Nestin protein.
Applications Notes
Paraformaldehyde-fixed, paraffin embedded (rat brain) ; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Nestin) Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C
Host or Source
Rabbit
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