Phospho-NeuroD1 (Ser274) Polyclonal Antibody

Product Specifications

Background

This gene encodes a member of the NeuroD family of basic helix-loop-helix (bHLH) transcription factors. The protein forms heterodimers with other bHLH proteins and activates transcription of genes that contain a specific DNA sequence known as the E-box. It regulates expression of the insulin gene, and mutations in this gene result in type II diabetes mellitus. [provided by RefSeq, Jul 2008]

CAS Number

9007-83-4

Structure Composition

0.01M TBS (pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Product Name Alternative

NeuroD1 (phospho S274) ; p-NeuroD1 (phospho S274) ; atonal; basic helix loop helix transcription factor; BETA 2; Beta cell E box transactivator 2; BETA2; BHF 1; BHF1; bHLHa3; class A basic helix loop helix protein 3; Class A basic helix-loop-helix protein 3; MODY 6; MODY6; NDF1_HUMAN; NEUROD; NeuroD1; Neurogenic differentiation 1; Neurogenic differentiation factor 1; neurogenic helix loop helix protein NEUROD; NIDDM

Swiss Prot

Q13562

Reactivity

Human, Mouse, Rat, Pig, Cow, Sheep

Immunogen

KLH conjugated Synthesised acetylpeptide derived from human NeuroD1 around the acetylation site of Ser274:PL (p-S) PP

Conjugation

Unconjugated

Applications

IHC-P

Dilution

IHC-P=1:100-500

Purity

Affinity purified by Protein A

Modification

Phosphorylation

Molecular Weight

~40 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -6°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

Phospho-NeuroD1 (Ser274) Polyclonal Antibody detects endogenous levels of NeuroD1 protein only when phosphorylated at Y274.

Applications Notes

Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue) ; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NeuroD1 (Ser274) ) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C