LW1/HSFX1 Polyclonal Antibody

Product Specifications

Background

HSFX1 (heat shock transcription factor family, X linked 1), also known as LW-1, is a 423 amino acid protein that is predominately expressed in testis. Localized to the cytoplasm, HSFX1 is thought to be involved in spermatogenesis and male fertility.

CAS Number

9007-83-4

Structure Composition

0.01M TBS (pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Product Name Alternative

HCG_27154; heat shock transcription factor family X linked 1; Heat shock transcription factor, X-linked; HSFX1; HSFX2; LW 1; LW1.

Swiss Prot

Q9UBD0

Reactivity

Human

Immunogen

KLH conjugated synthetic peptide derived from human LW1/HSFX1:181-280/423

Conjugation

Unconjugated

Applications

IHC-P

Dilution

IHC-P=1:100-500

Purity

Affinity purified by Protein A

Modification

Unmodification

Molecular Weight

~45 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -12°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

LW1/HSFX1 Polyclonal Antibody detects endogenous levels of LW1/HSFX1 protein.

Applications Notes

Paraformaldehyde-fixed, paraffin embedded (Human liver carcinoma) ; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HSFX1) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C