MSGN1 Polyclonal Antibody

Product Specifications

Background

Involved in the formation and segmentation of paraxial mesoderm. Has the regulatory capacity to specify ventrolateral (including future paraxial), but not dorsal (future axial), mesoderm phenotypes. Seems to be an upstream regulator of at least some elements of the Notch and Delta signaling pathway.

CAS Number

9007-83-4

Structure Composition

0.01M TBS (pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Product Name Alternative

Mesogenin-1 Mesogenin1; Msgn1; MSGN1_HUMAN; paraxial mesogenin.

Swiss Prot

A6NI15

Reactivity

Human, Mouse, Rat, Pig, Cow, Horse, Rabbit, Sheep

Immunogen

KLH conjugated synthetic peptide derived from human MSGN1:101-193/193

Conjugation

Unconjugated

Applications

IHC-P

Dilution

IHC-P=1:100-500

Purity

Affinity purified by Protein A

Modification

Unmodification

Molecular Weight

~21 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -23°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

MSGN1 Polyclonal Antibody detects endogenous levels of MSGN1 protein.

Applications Notes

Paraformaldehyde-fixed, paraffin embedded (mouse liver) ; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MSGN1) Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C