ZNF318 Polyclonal Antibody

Product Specifications

Background

ZNF318 is a 2279 amino acid endocrine regulatory protein that localizes to the nucleus. Highly expressed in testis, ovaries and kidneys, ZNF318 is a co-repressor of androgen receptor (AR) -mediated transcriptional activation and is thought to regulate transcription during spermatogenesis. ZNF318 interacts with the N-terminal domain of AR and contains two matrin-type zinc fingers. Two isoforms of ZNF318, designated TZF and TZF-L, are produced due to alternative splicing events. Each of these splice variants are thought to have unique roles in transcriptional regulation. While the TZF isoform functions as a repressor of AR-mediated transcriptional activation, the TZF-L isoform is thought to enhance AR-mediated transcriptional activation.

CAS Number

9007-83-4

Structure Composition

0.01M TBS (pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Product Name Alternative

Endocrine Regulatory Protein; HGNC13578; HRIHFB2436; ZFP318; Zinc Finger Protein 318; ZN318_HUMAN.

Swiss Prot

Q5VUA4

Reactivity

Human, Mouse, Rat, Dog, Pig, Horse, Rabbit

Immunogen

KLH conjugated synthetic peptide derived from human ZNF318

Conjugation

Unconjugated

Applications

WB, IHC-P

Dilution

WB=1:500-2000 IHC-P=1:100-500

Purity

Affinity purified by Protein A

Modification

Unmodification

Molecular Weight

125 kD

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

ZNF318 Polyclonal Antibody detects endogenous levels of ZNF318 protein.

Applications Notes

Sample: Lymph node (Mouse) Lysate at 40 ug Testis (Mouse) Lysate at 40 ug A549 (Human) Cell Lysate at 30 ug HL60 (Human) Cell Lysate at 30 ug 293T (Human) Cell Lysate at 30 ug Primary: Anti- ZNF318 at 1/1000 dilution