RRAGA + RRAGB Polyclonal Antibody

Product Specifications

Background

Involved in the RCC1/Ran-GTPase pathway. RRAGA may play a direct role in a TNF-alpha signaling pathway leading to induction of cell death. May alternatively act as a cellular target for adenovirus E3-14.7K, an inhibitor of TNF-alpha functions, thereby affecting cell death. Has guanine nucleotide-binding activity but undetectable intrinsic GTPase activity. biquitously expressed with highest levels of expression in skeletal muscle, heart, and brain.

CAS Number

9007-83-4

Structure Composition

0.01M TBS (pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Product Name Alternative

FIP1; RAGA; FIP-1; RRAGB; Adenovirus E3 14.7 kDa-interacting protein 1; Rag A; RAg B; RagA; RAGB; Ras related GTP binding protein A; RRAGA_HUMAN; Ras-related GTP binding A; GTP-binding protein ragB; Rag B; RagB; Ras-related GTP-binding protein B; RRAGB; RRAGB_HUMAN..

Swiss Prot

Q7L523

Reactivity

Human, Mouse, Rat, Dog, Pig, Horse, Sheep

Immunogen

KLH conjugated synthetic peptide derived from human RRAGA and RRAGB

Conjugation

Unconjugated

Applications

IHC-P

Dilution

IHC-P=1:100-500

Purity

Affinity purified by Protein A

Modification

Unmodification

Molecular Weight

37kD

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

RRAGA + RRAGB Polyclonal Antibody detects endogenous levels of RRAGA + RRAGB protein.

Applications Notes

Paraformaldehyde-fixed, paraffin embedded (mouse brain) ; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (RRAGA + RRAGB) Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C