Bcl-2 Polyclonal Antibody

The Bcl-2 gene was isolated at the chromosomal breakpoint of t (14;18) -bearing follicular B cell lymphomas (1,2) .Bcl-2 blocks cell death following a variety of stimuli and confers a death-sparing effect to certain hematopoietic cell lines following growth factor withdrawal (3,5) .Bcl-2 appears to function in several subcellular locations yet lacks any known motifs that would confer insight into its mechanism of action (6,7) .A more recently identified protein, designated Bax p21 (i.e., Bcl-associated X protein), has extensive amino acid homology with Bcl-2 and both homodimerizes and forms heterodimers with Bcl-2 (8) . Overexpression of Bax accelerates apoptotic death induced by cytokine deprivation in an IL-3 dependent cell line and Bax also counters the death repressor activty of Bcl-2 (8) .

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Western blot analysis of BCL-2 expression in SHSY5Y (A), MCF7 (B), Hela (C), mouse kidney (D), mouse muscle (E), rat kidney (F), rat muscle (G) whole cell lysates.