Bcl-2 Polyclonal Antibody
Product Specifications
Background
The Bcl-2 gene was isolated at the chromosomal breakpoint of t (14;18) -bearing follicular B cell lymphomas (1,2) .Bcl-2 blocks cell death following a variety of stimuli and confers a death-sparing effect to certain hematopoietic cell lines following growth factor withdrawal (3,5) .Bcl-2 appears to function in several subcellular locations yet lacks any known motifs that would confer insight into its mechanism of action (6,7) .A more recently identified protein, designated Bax p21 (i.e., Bcl-associated X protein), has extensive amino acid homology with Bcl-2 and both homodimerizes and forms heterodimers with Bcl-2 (8) . Overexpression of Bax accelerates apoptotic death induced by cytokine deprivation in an IL-3 dependent cell line and Bax also counters the death repressor activty of Bcl-2 (8) .
CAS Number
9007-83-4
Structure Composition
1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Apoptosis regulator Bcl-2
Swiss Prot
P49950
Reactivity
Human, Mouse, Rat
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human BCL-2.
Conjugation
Unconjugated
Applications
WB IHC IF/ICC
Dilution
WB: 1:500~1:1000 IHC: 1:50~1:200 IF/ICC: 1:50~1:200
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodified
Molecular Weight
28 kD
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
Bcl-2 Polyclonal Antibody detects endogenous levels of Bcl-2 protein.
Applications Notes
Western blot analysis of BCL-2 expression in SHSY5Y (A), MCF7 (B), Hela (C), mouse kidney (D), mouse muscle (E), rat kidney (F), rat muscle (G) whole cell lysates.
Host or Source
Rabbit
Available Sizes
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