Phospho-p53BP1 (Ser25 + Ser29) Polyclonal Antibody
Product Specifications
Background
P53 binding protein 1 (53BP1) plays a critical role in tumor suppression and is a putative substrate of ATM kinase. Upon DNA damage, it is phosphorylated and relocalizes to the presumptive sites of damage, specifically, double strand breaks. This also suggests a role in DNA repair, maintaining genomic stability.
CAS Number
9007-83-4
Structure Composition
0.01M TBS (pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Product Name Alternative
53BP1 (phospho S25) ; p-53BP1 (phospho S25) ;53BP1 (Phospho-Ser25/29) ; 53BP1 (Phospho-S25/S29) ; phospho-p53BP1 (Ser25/Ser29) ; P-53BP1 (Ser25/29) ; 53 BP1; FLJ41424; MGC138366; p202; p53 binding protein 1; p53 BP1; p53BP1; TP53 BP1; TP53BP1; Tumor protein 53 binding protein 1; Tumor protein p53 binding protein 1; Tumor suppressor p53 binding protein 1; TP53B_HUMAN; tumor protein 53-binding protein, 1.
Swiss Prot
Q12888
Reactivity
Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit
Immunogen
KLH conjugated Synthesised phosphopeptide derived from human p53BP1 around the phosphorylation site of Ser25/29:ED (p-S) QPE (p-S) QV
Conjugation
Unconjugated
Related Pathways
Cell Cycle G2 M DNA Damage Signaling Pathway
Applications
IHC-P
Dilution
IHC-P=1:100-500
Purity
Affinity purified by Protein A
Modification
Phosphorylation
Molecular Weight
213kD
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
Phospho-p53BP1 (Ser25 + Ser29) Polyclonal Antibody detects endogenous levels of phospho-p53BP1 protein only when phosphorylated at Ser25 + Ser29.
Applications Notes
Paraformaldehyde-fixed, paraffin embedded (Rat brain) ; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phospho-p53BP1 (Ser25 + Ser29) ) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C
Host or Source
Rabbit
Available Sizes
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