KLF2 Polyclonal Antibody
Product Specifications
Background
Kruppel-like factors (KLFs) are a family of broadly expressed zinc finger transcription factors. KLF2 regulates T-cell trafficking by promoting expression of the lipid-binding receptor S1P1 (S1PR1; MIM 601974) and the selectin CD62L (SELL; MIM 153240) (summary by Weinreich et al., 2009 [PubMed 19592277]) .[supplied by OMIM, Feb 2011]
CAS Number
9007-83-4
Structure Composition
0.01M TBS (pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Product Name Alternative
Kruppel like factor 2; Kruppel like factor; Kruppel like factor LKLF; Kruppel-like factor 2 (lung) ; Lung krueppel like factor; Lung Kruppel like zinc finger transcription factor; KLF 2; Klf2; KLF2_HUMAN; Krueppel-like factor 2; Lklf; Lung krueppel like factor; Lung krueppel-like factor; Lung Kruppel like zinc finger transcription factor.
Swiss Prot
Q9Y5W3
Reactivity
Human, Mouse, Rat, Pig, Cow, Horse, Rabbit, Sheep
Immunogen
KLH conjugated synthetic peptide derived from human KLF2:251-355/355
Conjugation
Unconjugated
Applications
ICC
Dilution
ICC=1:100
Purity
Affinity purified by Protein A
Modification
Unmodification
Molecular Weight
37kD
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
KLF2 Polyclonal Antibody detects endogenous levels of KLF2 protein.
Applications Notes
Tissue/cell: Mouse embryos;4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Incubation:Anti-KLF2 Polyclonal Antibody, Unconjugated 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugatedused at 1:200 dilution for 40 minutes at 37°C. DAPI (5ug/ml, blue, ) was used to stain the cell nuclei
Host or Source
Rabbit
Available Sizes
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